A BASKET SAMPLING TECHNIQUE FOR QUANTIFYING RIVERINE MACROBENTHOS1

ABSTRACT: A colonization basket sampling technique for quantifying macroinvertebrates in rubble bottom rivers is described. Basket samplers were compared to collections made using the Surber square foot sampler. Procedure for processing samples is described. Macrobenthos were more accurately quantified by basket samplers because both surface and subsurface habitats were sampled, no organisms were lost when samplers were removed from the river, substrata and associated fauna were retained for analysis, and the samplers were used efficiently in both pool and riffle areas. At the end of the colonization period the basket samplers contained one-half cubic foot of river bottom and macroinvertebrates spatially arranged within.     

溪流底栖动物定量与半定量采样法比较研究

溪流底栖动物定量和半定量采样法在个体数、物种数、物种相似性及生物指数方面的比较研究表明:(1)急流生境中,半定量样(踢网)的个体和物种数高于定量样(索伯网);静水-缓流生境中,半定量样(D形网)的个体和物种数一般高于定量样,且物种数有显著差异(z=-2.032,P<0.05).(2)同一样点半定量样(踢网加D形网)与定量样之间的物种相似性(平均为0.68)高于急流生境(0.56)和静水-缓流生境(0.45).(3)同一样点半定量样和定量样单独计算的生物指数值之间无显著差异.建议在应用溪流底栖动物开展水质生物评价时,可用半定量采样法完成野外采样.图4表1参8     

德兴铜矿废水对乐安江底栖动物群落的影响

对乐安江水系的底栖动物及其生活环境进行的研究表明，位于乐安江上游的德兴铜矿排出的大量酸性废水对主活在该水系中的底栖动物的分布及其群落结构会产生直接影响．废水中的各种污染物质，尤以重金属（特别是铜和铅、锌、镉）对底栖动物群落的抑制作用最大．在下游，随着同污染源距离的增加，河流的污染状况逐渐减轻，底栖动物种类多样性逐渐增加，清洁种类逐渐恢复．另外，河流水量的季节性变化对河流自身的净化作用会产生很大影响．     

Northern Rivers Ecosystem Initiative: Nutrients and Dissolved Oxygen – Issues and Impacts

Anthropogenic inputs of nitrogen (N), phosphorus (P) and oxygen-consuming material to aquatic ecosystems can change nutrient dynamics, deplete oxygen, and change abundance and diversity of aquatic plants and animals. The Northern Rivers Ecosystem Initiative required a research and assessment program to establish the contribution of pulp mill and sewage discharges to eutrophication and depressions in dissolved oxygen (DO) in the Athabasca and Wapiti rivers of northern Alberta, Canada and examine the adequacy of existing guidelines for protecting these systems. Analysis of long-term data showed that total N (TN) and total P (TP) concentrations in exposed river reaches exceeded concentrations in reference reaches by ≤ 2 times for the Athabasca River, and by 9.6 (TP) and 2.6 (TN) times for the Wapiti River. Results from nutrient limitation experiments conducted in situ and in mesocosms showed that benthic algal production was nutrient sufficient downstream of pulp mill discharges but constrained in upper river reaches by insufficient P (Athabasca River) or N + P (Wapiti River). Dissolved oxygen (DO) concentrations in both rivers declined during winter such that median concentrations in the Athabasca River 945 km downstream of the headwaters were approximately 8 mg L⁻¹ in mid-February. Although water column DO rarely approached the guideline of 6.5 mg L⁻¹, DO studies undertaken in the Wapiti River showed that pore water DO often failed to meet this guideline and could not be predicted from water column DO. Results from this integrated program of monitoring and experimentation have improved understanding of the interactions between nutrients, DO and aquatic ecosystem productivity and resulted in recommendations for revisions to nutrient and DO guidelines for these northern rivers.The Canadian Crown reserves the right to retain a non-exclusive, royalty free licence in and to any copyright.     

Variability in the identification and enumeration of marine benthic invertebrate samples and its effect on benthic assessment measures

Studies designed to measure anthropogenic impacts on marine benthic communities depend on the ability of taxonomists to consistently discriminate, identify, and count benthic organisms. To quantify errors and discrepancies in identification and enumeration, 20 samples were completely reprocessed by another one of four participating laboratories. Errors were detected in 13.0% of the data records, affecting total abundance by 2.1%, numbers of taxa by 3.4%, and identification accuracy by 4.7%. Paired t-tests were used to test for differences in the Benthic Response Index (BRI), total abundance, numbers of taxa, and the Shannon-Wiener index between the original and the reanalysis data. Differences in the BRI were statistically insignificant. Although statistically significant differences were observed for numbers of taxa, total abundance, and the Shannon-Wiener index, the differences were small in comparison to the magnitude of differences typically observed between anthropogenically affected and reference sites.     

Spatial scales and probability based sampling in determining levels of benthic community degradation in the Chesapeake Bay

The extent of degradation of benthic communities of the Chesapeake Bay was determined by applying a previously developed benthic index of biotic integrity at three spatial scales. Allocation of sampling was probability-based allowing areal estimates of degradation with known confidence intervals. The three spatial scales were: (1) the tidal Chesapeake Bay; (2) the Elizabeth River watershed; and (3) two small tidal creeks within the Southern Branch of the Elizabeth River that are part of a sediment contaminant remediation effort. The areas covered varied from 10^–1 to 10⁴ km² and all were sampled in 1999. The Chesapeake Bay was divided into ten strata, the Elizabeth River into five strata and each of the two tidal creeks was a single stratum. The determination of the number and size of strata was based upon consideration of both managerially useful units for restoration and limitations of funding. Within each stratum 25 random locations were sampled for benthic community condition. In 1999 the percent of the benthos with poor benthic community condition for the entire Chesapeake Bay was 47% and varied from 20% at the mouth of the Bay to 72% in the Potomac River. The estimated area of benthos with poor benthic community condition for the Elizabeth River was 64% and varied from 52–92%. Both small tidal creeks had estimates of 76% of poor benthic community condition. These kinds of estimates allow environmental managers to better direct restoration efforts and evaluate progress towards restoration. Patterns of benthic community condition at smaller spatial scales may not be correctly inferred from larger spatial scales. Comparisons of patterns in benthic community condition across spatial scales, and between combinations of strata, must be cautiously interpreted.     

A More Cost-Effective Emap Benthic Macrofaunal Sampling Protocol

Benthic macrofaunal sampling protocols in the U.S. Environmental Protection Agency's Environmental Monitoring and Assessment Program (EMAP) are to collect 30 to 50 random benthic macrofauna [defined as animals retained on a 0.5 mm (East and Gulf Coasts, USA) or a 1.0 mm mesh sieve (West Coast, USA)] samples per reporting unit using a 0.044 m² (East and Gulf Coasts) or 0.1 m² (West Coast) grab. Benthic macrofaunal community conditions in the reporting unit are characterized by cumulative distribution functions (CDFs) on end points of interest, such as number of species (S), abundance (A), and Shannon--Wiener diversity (H′). An EMAP and a companion field study were conducted concurrently in Tillamook Bay (Oregon, USA) to compare the cost effectiveness of benthic macrofauna samples collected using the EMAP West Coast (0.1 m² × ≥7 cm deep, 1.0 mm mesh), a 0.01 m² × 5 cm deep, 1.0 mm mesh, and a 0.01 m² × 5 cm deep, 0.5 mm mesh sampling protocol. Cost was estimated in relative laboratory sample-processing time. Sampling protocols were judged equally effective for EMAP purposes if, after linear transformation to adjust for scale changes in end point distributions, their S, A, and H′ CDFs were not significantly different. The 0.01 m² × 5 cm deep, 1.0 mm mesh sampling protocol was the most cost effective.     

COMPARISON OF FOUR ARTIFICIAL SUBSTRATES AND THE PONAR GRAB FOR BENTHIC INVERTEBRATE COLLECTION1

ABSTRACT: Four different bottom-placed artificial substrates were compared with the Ponar grab for collecting benthic invertebrates. Artificial substrate samples of organisms were larger and more diverse than those of the grab. Barbeque Basket samplers caught the most taxa and individuals and Beak Trays caught the least. Chironomids and crustaceans were dominant in artificial substrate samples. Exposure habitat (left or right bank) determined taxa availability, whereas sampler design determined suitability for colonization by the taxa. Diversity for Beak Tray samples was lower than that for other artificial substrates but higher than for Ponar samples. The Barbeque Basket, Bull Basket, and Multiple Plate samples were taxonomically similar. Ponar samples were different, and Beak Trays were of intermediate similarity. As qualitative samplers, Barbeque Baskets were 63 percent efficient, followed by Bull Baskets (55 percent), Multiple Plates (48 percent), Beak Trays (38 percent), and Ponar Grabs (6–10 percent). Bull Baskets required the least and Beak Trays the most replicates to be within a preselected percentage error of the mean at the 95 percent probability level for numbers of taxa and individuals, and for diversity. Under conditions of the study, Bull Baskets ranked highest, followed by Barbeque Baskets and Multiple Plates, in selected performance criteria. Differences between grab and artificial substrate samples are explainable in terms of major riverine habitats and characteristics of the collection methods.     

BENTHIC INVERTEBRATES IN A NORTH-FLOWING STREAM AND A SOUTH-FLOWING STREAM,BROOKS RANGE,ALASKA1

ABSTRACT: Benthic invertebrate faunas were compared to two fifth-order streams, the Atigun River flowing northward and the Dietrich River flowing southward. Sixty-eight taxa were collected, forty-nine from each stream. Aquatic insects comprised 88% of the taxa and 97% of the individuals from the Dietrich River and 73% of the taxa and 97% of the individuals from the Atigun River. Diptera, especially Chironomidae, were most abundant. Plecoptera, Ephemeroptera, Oligochaeta, Acarina, and Collembola were significant. In both streams the headwaters were dominated by the subfamily Diamesinae which was replaced by Orthocladiinae downstream. Diversity seemed to increase with stream order. Cluster analysis showed a high degree of resemblance between the benthic faunas of the rivers. Faunal resemblance decreased with increasing distance between stations, both within and between the streams. Although some taxa may occur in only one stream, the evidence for faunal resemblance is stronger than for faunal differences. Other studies have shown that differences in total radiation associated with valley aspect affect local climate, hydrology, and distribution of terrestrial plants and animals. However, the benthic faunas of Atigun and Dietrich Rivers were remarkably similar. Factors which operate independently of aspect, possibly freezing solid in winter, may control the occurrence of species in these streams.     

长江支流桥边河大型底栖动物群落结构及水质生物评价

中小河流数量众多,分布广泛,其健康状况将直接影响和决定着大江大河的生态系统健康,如何准确、合理的评估中小河流的健康状况,已经成为世界性的科学问题.本研究以长江一级支流桥边河为例,于2019年1月（枯水期）和8月（丰水期）在河道的上中下游设置8个采样点,进行桥边河大型底栖动物群落结构调查,利用Shannon-Wiener多样性指数、耐污值法和科级生物指数法（FBI）分别对各样点进行水质生物评价.调查结果显示,枯水期采集到底栖动物17个分类单元,共283个底栖动物;丰水期采集到16个分类单元,共301个底栖动物,丰水期大型底栖动物个体数略高于枯水期.水质生物评价结果显示,桥边河水质状况时空差异性明显,丰水期的水质状况优于枯水期,空间尺度上丰枯水期水质状况均为上游段 > 中游段 > 下游段.3种水质生物评价法对比分析显示,FBI科级生物指数的评价结果与实际情况更吻合,更适合桥边河水质评价,其研究结果可为中小河流生态系统健康评价提供参考.